2X CANINE TICK-BORNE DISEASE (EHRLICHIA–LYME-ANAPLASMA-BABESIA) TEST KIT

Description

Introduction to Tick-borne Disease:
Tick-borne disease occurs when ticks infected with a pathogen bite a dog and transmit the pathogen into the dog's body. Many of these pathogens are zoonotic, meaning they can also infect humans. Disease is not spread between dogs and humans directly because these pathogens must complete their lifecycle phase within the tick to become infectious. Therefore, while humans and other non-canine family members can also become infected, a direct tick bite is required to transmit disease. The most common tick-borne diseases are Ehrlichiosis, Anaplasmosis, Rocky Mountain spotted fever, Babesiosis, and Lyme disease. The feeding time required to allow disease transmission from a tick to a dog or person varies between ticks and disease agents. Ehrlichiosis and Rocky Mountain Spotted Fever-causing bacteria can be transmitted within 3-6 hours of tick attachment, while Lyme Disease-causing bacterial transmission can require 24-48 hours of feeding before a host is infected.
Intended use:
Ehrlichia – Lyme - Anaplasma - Babesia Combo rapid Test kit is a raid Test to diagnose the presence of tick-borne diseases antibodies against Ehrlichia canis, Ehrlichia ewingii, Borrelia burgdorferi, Anaplasma phagocytophilum and Anaplasma platys , and babesia in dog’s serum or plasma specimen.
Assay Time
:
5-10 minutes
Specimen:
Serum, plasma
Principle:
The Ehrlichia – Lyme - Anaplasma - Babesia Combo Test is based on sandwich lateral flow Immunochromatographic assay.
Storage and stability:
- The kit can be stored at room temperature (4-30°C).
- The test kit is stable
Expiration date:
10/10/2022
Specimen preparation and storage:
- Specimen should be obtained and treated as below.
- Serum or plasma: collect the whole blood from your dog, centrifuge it to get the plasma, or place the whole blood into a tube which contains anticoagulants to get serum.
- All specimen should be tested immediately. If not for testing right now, they should be stored at 2-8℃.
Test procedure:
1- Allow all materials, including Test strip to recover to 15-25℃ before running the assay.
2- Draw 1.5-2mL blood from your dog.
3- let the blood tube in room temperature for 10 to 15 minutes for blood coagulation.
4- place the tube in the Centrifuge and spin for 5 to 7 minutes at 4000rpm or at higher speed If there is no centrifuge available, after collecting the blood, keep the blood tube (plain Tube) in a standing position for 8-10 hours at 2-8C (in the refrigerator) then aspirate 1 drop of the Serum and add into the assay diluent buffer and mix thoroughly.
5- Take out the test strip from the foil pouch and place it horizontally.
6- aspirate one drop (50ul) of Serum and add it into the assay diluent buffer.
7- mix thoroughly for a few seconds.
8- aspirate 3 drops (150ul) of the diluent sample.
9- add the 3 drops of the diluent sample (serum/assay mixture) into the sample holes/Test wells "S" of the Test strip (add 3 drops of serum/assay mixture in each of the 4 small holes/Test wells of the Test strip)
10- Interpret the result at 5-10 minutes. Result after 10 minutes is considered invalid.
Interpretation of the Test result:
- Positive (+):
The presence of both “C” line and zone “T” line, no matter T line is clear or vague.
- Negative (-):
Only clear "C" line appear. No "T" line.
- Invalid:
No colored line appears in "C" zone. No matter if "T" line appears.
Precautions:
- All reagents must be at room temperature before running the assay.
- Do not remove Test strip from its pouch until immediately before use.
- Do not use the test beyond its expiration date.
- The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot.
Test package includes:
- 1x Test kit
- 1x dropper
- 1x assay diluent
- 1x 1.5ml Tube
Contact us:
If for any reason you feel you cannot leave us a positive 5 stars feedback, please contact us so we can work this out. Please message us before leaving feedback or opening a case that way we can do our best to resolve any issue you have. Customer satisfaction is our priority; we want to make more deals with you.
Please
Check out our store formore Canine wellness Test Kits:
- Canine and Feline RLX  pregnancy Test kit.
- Canine Brucellosis Ab Raid Test Kit.
- Canine IgE Rapid Test Kit (Canine Allergy Indicator)
- Canine Distemper Virus Antibody Rapid Test Kit (CDV Ab Titer Test)
- Canine Parvo Virus Antibody Rapid Test kit (CPV Ab Titer Test)
- Canine Rabies Ab Rapid Test Kit (TITER Test)
- Canine H-W Ag Rapid Test Kit.
Please feel free to message us with any further question. Thank you for your order!